A research platform for miRNA-based treatment of multiple myeloma and chronic lymphoctic leukemia

MicroRNAs (miRNAs) are a class of small non-coding RNAs which regulate gene
expression at the post-transcriptional level. miRNAs play a role in tumorigenesis acting as
oncogenes or tumor suppressor genes. Aberrant miRNA expression is associated to
prognosis and therapeutical response. Deregulation of miRNA expression is involved in
the pathogenesis of chronic lymphocytic leukemia (CLL) and multiple myeloma (MM)
where miRNAs are important therapeutic targets. Two approaches can be theoretically
used to achieve a therapeutic effect by targeting the miRNA regulatory network: i) enforce
the activity of anti-tumor miRNAs (miRNAs as drug), ii) inhibit the activity of pro-tumor
miRNAs (miRNAs as target) by means of antagomirs.
The aim of our project is to exploit miRNAs as drugs or targets in innovative therapeutic
strategies for CLL and MM using novel in vitro and in vivo preclinical models and delivery
nanotechnologies. We propose an experimental platform which includes all steps from
target identification to FIRST in HUMAN studies. In Task 1, miRNAs, whose expression is
consistently deregulated in CLL and MM, as well as in non-tumor cells of the tumor bone
marrow microenvironment (BMM), will be defined by molecular profiling of clinical samples
from the framework of nationwide clinical trials. In Task 2, we will evaluate the effects
induced by targeting deregulated miRNAs on cell survival/proliferation and on selected
pathways using molecular profiling technologies.    Task 3 will be devoted to the design,
preparation,   characterization   and   evaluation   of   liposomes,   supramolecular   lipidic
aggregates (SLAs) and colloidal polymeric carriers for in vivo delivery of engineered
miRNAs or antagomirs. Specifically, we will attempt to functionalize nanocarrier surfaces
by humanized mAbs against anti-CLL and -MM-associated antigens. Reagents produced
in this Task will be tested in murine models of CLL and MM (Task 4). The investigation will
take benefit of the E-µu-TCL1 transgenic mouse model of human CLL and of a NOD/SCID
mouse model for in vivo expansion of CLL cells. Moreover, we will use the SCID-hu model
of human MM and an innovative in vivo system (SCID-synth-hu) based on a 3-D bone-like
poly-ε-caprolactone biopolymeric scaffold coated in the 3-D space with BMSCs, which
allow the growth of primary MM cells in human fetal or adult autologous BMM,
respectively. Task 5 will afford the clinical grade production of selected bioreagents for
preclinical   pharmacokinetics   and   toxicology   in   animals   in   order   to   achieve   an
investigational new drug status. These miRNAs/antagomirs carrying nanovectors will
finally proceed to early clinical trials (Phase 0-I) for FIRST in HUMAN evaluation (Task 6).
The possibility of in vivo monitoring of effective nanovector delivery   with unique
sophisticated preclinical models as well as the chance of real-time monitoring of patients
undergoing early clinical trials will provide an array of opportunities for re-designing the
molecular reagents and improve delivery strategies. The design of this project as a
platform will provide the best information exchange among investigators and will allow re-
direction of the experimental flow on the basis of available information in order to achieve
the final goal of new treatments for CLL and/or MM by a timely translational network.

Principal Investigators:

ANTONINO NERI

Financing institution:

FONDAZIONE AIRC PER LA RICERCA SUL CANCRO ETS

Type:

ASS_NAZ - Bandi da Associazioni e altri Finanziatori privati nazionali

Project leader:

UNIVERSITA' DEGLI STUDI MAGNA GRECIA DI CATANZARO

Year:

2010

Duration:

Status:

Closed