Evaluation of the use of keratinocytes sheets and ex vivo gene therapy in the treatment of epidermolysis bollosa
The aim of the study is the production of neo-skin formed by sheets of keratinocytes and / or fibroblasts treated by ex vivo gene therapy for the potential treatment of patients with epidermolysis bullosa.
The execution of this sperimentation would lead solid foundation for the treatment of a severely disabling disease from the perspective of both organic and social.
The study is divide into five main phases:
- phase of biopsy on patients with epidermolysis bullosa, for the selection of autologous keratinocytes / fibroblasti
- phase of gene therapy through retroviral carrier
- phase of amplification through keratinocytes / fibroblasti culture in vitro
- phase of histological analysis of the results